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KMID : 1171820050200030010
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Journal of Environmental and Sanitary Engineering
2005 Volume.20 No. 3 p.10 ~ p.16
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Effect of Chestnut Bark Extracts on Tyrosinase Gene Expression
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Chin Jong-Eon
Kim Kwan-Chun
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Abstract
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Chestnut bark extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. 10 ㎍/㎖, 100 ㎍/㎖, 1 ㎎/㎖ of the extract repressed expression of tyrosinase gene about 38%, 47%, and 78%, respectively, compared with control. In the MTT assay, the same extract exhibited very low cytotoxicity at 1 ㎍/㎖, 10 ㎍/㎖, 100 ㎍/㎖, and 1 ㎎/㎖, respectively. The fractions of Methylene chloride and ethyl acetate did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of butyl alcohol repressed highly at 10 ㎍/㎖ and 100 ㎍/㎖.
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KEYWORD
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Chestnut Bark, Tyrosinase Gene, B16 mouse melanoma cell
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